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Expression of serotonin, sensory neuropeptides, tachykinin receptors and innervation in contact allergy: kinetic and functional studies
Huvudsökande: Husameldin El-Nour
Arbetsplats: Dermatology and Venereology Unit, Karolinska University Hospital, Solna
Allergic contact dermatitis (ACD) is a common inflammatory skin disorder affecting 20% of the population. There is increasing evidence of neuroimune interactions in ACD. The mechanisms of involvement of the serotonergic system, nerve fibres and the sensory neuropeptides (substance P and neurokinin A) have been studied in skin biopsies from nickel conatct allergic patients, obtained at 0, 6, 24, 48 and 72 h, post-challenge with nickel sulphate. The cross-talk between the serotonergic system and immune system focusing on T-regulatory cells and cytokines will be studied in these biopsies. A role for the selective serotonin reuptake inhibitor (citalopram) in murine allergic contact dermatitis is studied using female Balb/C mice. Furthermore, the mechanisms by which a citalopram-mediated effect on murine contact allergy are investigated. This includes analysis and quantification of expression of the serotonin ligand and receptros as well as analysis of substance P expression in murine contact allergic skin. In addition, the concentrations of these protein are measured using radioimmunoassay. The outcome of this project work will increase our understanding of the neuroimmune mechanisms in inflammatory skin conditions and ACD. It may also provide basic information for a future therapeutic approach to treat contact dermatitis via targeting the studied neuromediators.